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KMID : 1007519980070010028
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Food Science and Biotechnology
1998 Volume.7 No. 1 p.28 ~ p.34
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Simultaneous Determination of Glucose , Lactate and Cholesterol Using an Oxygen Electrode with the Multiple Cathode System
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Kim, Jung Ho
Rhie, Dong Hee/Kim, Tai Jin/Noh, Bong Soo
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Abstract
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The purpose of this study was to develop a biosensor for rapid and accurate analysis of glucose, lactate and cholesterol in food-stuffs and to enhance convenience and automation of the analytical system, resulting in the establishment of biomonitoring techniques on various food components. A multiple cathode system was prepared with an oxygen electrode having one anode and multiple cathodes. Three components were simultaneously determined by three immobilized enzymes with the multiple cathode system. Glucose oxidase, lactate oxidase, and cholesterol oxidase and cholesterol esterase were respectively immobilized for the glucose, lactate and cholesterol multiple biosensor system. The concentration of components determined by enzyme sensors were based on the maximum slope of oxygen consumption from enzyme reaction. The response time for each analysis was about 1 min. The optimum condition for simultaneous determination was found to be 0.1 M potassium phosphate buffer, pH 7.0 at 40℃. Effects of various interferences such as sugars, organic acids, and triglyceride on this system were investigated. Less than 8% of error was found in determination of lactate and cholesterol. The lactate interference at glucose sensor was compensated by a modified equation. This system was confirmed by a conventional methods. It was concluded that the multiple cathode system of this study was used for an efficient method to determine sugar, organic acid, and lipid simultaneously in foods.
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